Liquid–liquid extraction method using a mixture of icecold methanol and ultrapure deionised MiLiQ water was performed on the samples. 29 Briefly, 800 µL of icecold mixture containing HPLCgrade methanol and ultrapure deionised MiLiQ water at a ratio of (8:1) (v/v) was mixed with 100 µL of serum, vortexed for 2 min and stored at 4°C.
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